منابع مشابه
The reactivity and function of thiol groups in trout actin.
1. Considerable differences were found between the rates and degrees of modification of native trout actin with iodo[2-(14)C]acetate and iodo[1-(14)C]acetamide. 2. With iodoacetate, G- and F-actin were both labelled in the N-terminal peptide only. This modification had little effect on the ability of the actin to polymerize. 3. Iodoacetamide labelled three cysteine residues in both G- and F-act...
متن کاملThe thiol groups of fumarase.
Several reagents have been employed to determine the role of the thiol groups in fumarase. The nature of the reaction of these reagents indicates that the thiol groups are not associated with active site structures but are buried in a hydrophobic environment in the interior of the enzyme. Several observations are in accord with this view. The rate of reaction of the thiol groups is much slower ...
متن کاملHuman tryptophan transfer ribonucleic acid synthetase. Composition, function of thiol groups, and structure of thiol peptides.
Human tryptophanyl-tRNA synthetase resembles its counterpart in Escherichia coli in quaternary structure (alpha2), but differs in molecular weight, amino acid composition, the number of thiol groups, and the relationship of the thiol groups to enzyme activity. Nevertheless, one of the thiol groups resides in a heptapeptide sequence homologous to a heptapeptide sequence containing a thiol group ...
متن کاملThe pH 6 acetolactate-forming enzyme from Aerobacter aerogenes. Determination and function of thiol groups.
Kinetic studies have been performed with a recrystallized preparation of the pH 6 acetolactate-forming enzyme from Aerobacfer aerogenes. The pH optimum in acetate is 5.8, and the enzyme activity is inhibited by phosphate and sulfate. These compounds cause sigmoidal kinetics, and apparent Michaelis kinetics is observed in acetate. The enzyme is sensitive to inhibition by the substrate analogues,...
متن کاملPathways of peroxynitrite oxidation of thiol groups.
Peroxynitrite mediates the oxidation of the thiol group of both cysteine and glutathione. This process is associated with oxygen consumption. At acidic pH and a cysteine/peroxynitrite molar ratio of < or = 1.2, there was a single fast phase of oxygen consumption, which increased with increasing concentrations of both cysteine and oxygen. At higher molar ratios the profile of oxygen consumption ...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1970
ISSN: 0306-3283
DOI: 10.1042/bj1170061p